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Functional Analysis

Functional Analysis

Experience unparalleled insights into antibody functionality with our cutting-edge suite of analysis tools, customized to your panel requirements


Assessing the performance and potential of your antibodies

Our team recognizes the critical importance of comprehensive antibody functional characterization for successful therapeutic development. We provide thorough evaluations of your antibodies, assessing the binding affinity characteristics to both soluble and membrane-bound targets. Furthermore, we investigate their mechanism of action and effector functions.

Each assay we perform is customized to your unique project requirements, enabling the selection of the best performing antibodies. With our services, you gain the confidence to move forward in your development process, assured in the knowledge that your chosen antibodies have been thoroughly validated and characterized.

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We incorporate Surface Plasmon Resonance (SPR) into our comprehensive antibody characterization. SPR offers real-time, label-free assessment of in vitro protein-protein interactions, allowing us to characterize and rank your antibody panel based on their binding to antigens. Our SPR capabilities extend to include affinity screening, epitope characterization, determination of specificity, mechanism of action investigations, and Fc receptor-binding studies. These detailed insights significantly aid in understanding an antibody potential functionality.

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Kinetics Affinity

Kinetic affinity determination is a crucial method for quantifying the strength and specificity of interactions between two biological molecules, often an antibody and its target antigen. This real-time tracking lets us measure the rates of association and dissociation, resulting on the binding affinity calculation. This method is essential in therapeutic development as it illuminates molecular recognition dynamics and helps evaluate potential therapeutic efficiency dynamics and helps evaluate potential therapeutic efficiency.

pH Dependent Binding

Using SPR, our pH-dependent assessment of molecular interactions takes into account how environmental conditions influence antibody-target or antibody-receptor bindings. This evaluation supports antibody engineering efforts, enhancing target-mediated antibody recycling and boosting the cellular uptake of the antibody-antigen complex into endosomes, thereby optimizing therapeutic effectiveness.

Epitope Binning Mapping

We utilize SPR as a key tool in epitope binning studies, helping us identify antibodies that recognize identical or closely related epitopes on a given antigen. The data generated from these studies is pivotal for understanding your antibody functional properties, guiding candidate selection, and offering a clear perspective on the diversity and comprehensive coverage of your antibody panel.

AlphaScreen® and AlphaLISA® assays

We utilizeAlphaScreen® and AlphaLISA® homogeneous assays in our antibody characterization to quantify diverse biomolecular interactions. Known for their sensitivity and versatility, these assays can detect a wide array of soluble and cellular analytes and assess interactions with varying affinity levels. Their applications extend beyond interaction assays, encompassing enzymatic assays, immunoassays, and even GPCR functional assays.

Cell-based Assays

At the core of our bioanalytical capabilities lies our proficiency in cell-based assays, essential for our tailored, advanced antibody characterization services. Our expert cell biologists utilizecutting-edge technology to optimize assay development, delivering key insights into antibody functions. We conduct an extensive range of in vitro functional assays, including binding specificity, cell killing, proliferation studies, antigen-mediated antibody internalization, neutralization, and Fc-mediated effector functions such as ADCP, ADCC, and CDC. These comprehensive assessments probe into antibody activity, efficacy, toxicity, and their mechanism of action, serving as a vital resource throughout the entire biologic development process, from initial discovery to IND submission.

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T cell activation

We specialize in cutting-edge T cell activation assays, analyzing key T cell responses, such as proliferation, cytokine production, and T cell-mediated cytotoxicity. Our approach provides deep insights into T cell functionality, which is essential for the development of T cell-targeted therapies. By understanding these dynamics, we enhance the potential effectiveness of treatments for challenging disorders, including cancer and autoimmune diseases.

Cell killing

We utilize specific methods to understand cell killing by immune cells, a process involving the targeted elimination by immune components such as cytotoxic T cells and natural killer (NK) cells. This mechanism enables the immune system to neutralize threats, playing an indispensable role in overall health maintenance. In the immune-mediated killing assays, target and effector cells are co-cultivated, and the consequent cell death is analyzed using techniques such as flow cytometry, providing valuable insights into the dynamics and effectiveness of the immune response.


Our advanced antibody internalization services provide quantitative assessment of cellular uptake of antibody-antigen complexes, supporting the development of antibody-based therapeutics, such as antibody-drug conjugates (ADCs). We specialize in quantitative assessment of the internalization of antibodies bound to specific cell surface antigens, crucial for their efficacy against antigen-expressing cancer cells.


Cell proliferation and viability are crucial indicators in assessing therapeutic outcomes and pharmacological safety during drug development. We offer extensive experience in evaluating the effects of antibodies on cell proliferation and viability using a comprehensive panel of technologies, including diverse cell lines and specialized reagents for assessing proliferation changes under specific conditions.


We offer specialized services in the assessment of mast cell degranulation using optimized in vitro methods, providing valuable insights into the mechanisms of action of antibodies and supporting therapeutic development in allergic and immune disorders.

Customized assay development

We prioritize your success by offering personalized and tailored approaches that are meticulously designed to meet your antibody’s unique biology and application. Our expert teams collaborate closely to develop custom functional assays aligned with your program needs. By maintaining a flexible approach, we ensure the accuracy and relevance of our assays, empowering informed decisions and efficient achievement of your research goals.

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