FunctionInsight Assays

FunctionInsight Assays

Turn a Binder into a Therapeutic

Finding an antibody that binds is a starting point. Knowing that it drives the right biological effect, in the right cellular system, through the right mechanism, is what determines whether it becomes a drug. FunctionInsight Assays delivers that evidence, from target validation through to development candidate selection, with custom and off-the-shelf functional assays designed around your molecule's specific biology.

Discuss your assay requirements with our team

Turn a Binder into a Therapeutic

Finding an antibody that binds is a starting point. Knowing that it drives the right biological effect, in the right cellular system, through the right mechanism, is what determines whether it becomes a drug. FunctionInsight Assays delivers that evidence, from target validation through to development candidate selection, with custom and off-the-shelf functional assays designed around your molecule's specific biology.

Discuss your assay requirements with our team

Turn a Binder into a Therapeutic

Finding an antibody that binds is a starting point. Knowing that it drives the right biological effect, in the right cellular system, through the right mechanism, is what determines whether it becomes a drug. FunctionInsight Assays delivers that evidence, from target validation through to development candidate selection, with custom and off-the-shelf functional assays designed around your molecule's specific biology.

Discuss your assay requirements with our team

Ensure Therapeutic Impact

Identify candidates that drive the desired biological effect in the relevant cellular systems — not just binders, but molecules with the functional profile needed to achieve the intended therapeutic mechanism.

Increase Probability of Success

Early functional insights improve predictability and accelerate decision-making. The right assay data at lead selection prevents the wrong candidates from advancing.

De-risk Development

Prioritising functional characterisation upfront minimises late-stage attrition, shortens overall timelines, and reduces the cost of failure. Functional liabilities discovered at lead selection are recoverable. Those discovered in the clinic are not.

Value chain positioning

FunctionInsight Assays is active at three critical decision points: Target Validation, Lead Selection, and Development Candidate Selection — covering the full span of functional questions from initial target biology through to the go/no-go candidate decision.

Explore the full discovery-to-IND offering

Explore discovery-to-IND

Whatever the Function, We Can Measure It.

Assay Portfolio

FunctionInsight Assays covers the complete spectrum of antibody functional readouts — from target-driven biology to immune system engagement — using custom and off-the-shelf assays designed around your molecule's specific biology.

Whatever the Function, We Can Measure It.

Assay Portfolio

MultiPath Discovery is built around three independent axes — source of diversity, discovery platform, and final molecular format. For every project, our scientific leads select the combination that gives your programme the best probability of delivering a clinical candidate.

Whatever the Function, We Can Measure It.

Assay Portfolio

MultiPath Discovery is built around three independent axes — source of diversity, discovery platform, and final molecular format. For every project, our scientific leads select the combination that gives your programme the best probability of delivering a clinical candidate.

Target binding-based functions  

  • Cells Binding and Kinetics — target engagement on cell surface-expressed targets, potency and binding kinetics in cellular context

  • Cell Signalling Activation — pathway activation downstream of target engagement

  • Internalisation — confirmation and quantification of antibody internalisation following target binding

  • Neutralisation — blocking of ligand-receptor interaction or functional pathway inhibition

  • Blocking / Competition — receptor occupancy, ligand blocking, competitive inhibition

  • Cellular Proliferation — anti-proliferative activity in relevant tumour or immune cell models

  • Cellular Apoptosis — apoptosis induction in target-expressing cell lines

  • T-cell Dependent Cellular Cytotoxicity (TDCC) — T-cell mediated killing of target-expressing cells

Target binding-based functions  

  • Cells Binding and Kinetics — target engagement on cell surface-expressed targets, potency and binding kinetics in cellular context

  • Cell Signalling Activation — pathway activation downstream of target engagement

  • Internalisation — confirmation and quantification of antibody internalisation following target binding

  • Neutralisation — blocking of ligand-receptor interaction or functional pathway inhibition

  • Blocking / Competition — receptor occupancy, ligand blocking, competitive inhibition

  • Cellular Proliferation — anti-proliferative activity in relevant tumour or immune cell models

  • Cellular Apoptosis — apoptosis induction in target-expressing cell lines

  • T-cell Dependent Cellular Cytotoxicity (TDCC) — T-cell mediated killing of target-expressing cells

Target binding-based functions  

  • Cells Binding and Kinetics — target engagement on cell surface-expressed targets, potency and binding kinetics in cellular context

  • Cell Signalling Activation — pathway activation downstream of target engagement

  • Internalisation — confirmation and quantification of antibody internalisation following target binding

  • Neutralisation — blocking of ligand-receptor interaction or functional pathway inhibition

  • Blocking / Competition — receptor occupancy, ligand blocking, competitive inhibition

  • Cellular Proliferation — anti-proliferative activity in relevant tumour or immune cell models

  • Cellular Apoptosis — apoptosis induction in target-expressing cell lines

  • T-cell Dependent Cellular Cytotoxicity (TDCC) — T-cell mediated killing of target-expressing cells

Fc-mediated effector functions  

  • ADCC — Antibody-Dependent Cell-Mediated Cytotoxicity

  • ADCP — Antibody-Dependent Cellular Phagocytosis

  • CDC — Complement-Dependent Cytotoxicity

  • FcγR Binding — full Fc gamma receptor panel

  • FcRn Binding — pH-dependent interaction for half-life strategy

Fc-mediated effector functions  

  • ADCC — Antibody-Dependent Cell-Mediated Cytotoxicity

  • ADCP — Antibody-Dependent Cellular Phagocytosis

  • CDC — Complement-Dependent Cytotoxicity

  • FcγR Binding — full Fc gamma receptor panel

  • FcRn Binding — pH-dependent interaction for half-life strategy

Fc-mediated effector functions  

  • ADCC — Antibody-Dependent Cell-Mediated Cytotoxicity

  • ADCP — Antibody-Dependent Cellular Phagocytosis

  • CDC — Complement-Dependent Cytotoxicity

  • FcγR Binding — full Fc gamma receptor panel

  • FcRn Binding — pH-dependent interaction for half-life strategy

Technologies

Flow Cytometry

Multiparametric, high-throughput analysis enabling simultaneous measurement of multiple functional and phenotypic parameters including cytokine bead arrays, across complex cellular systems.

SPR — Surface Plasmon Resonance

Real-time kinetic understanding of target engagement — kon, koff, KD — in biochemical and cell-based formats.

Reporter Gene Assays

Fast, quantitative functional readout using stable reporter cell lines (e.g. NF-κB-Luc) for high-throughput pathway activation and inhibition screening.

Cell Binding Kinetics

Potency and target engagement characterisation in biologically relevant cellular systems.

Technologies

Flow Cytometry

Multiparametric, high-throughput analysis enabling simultaneous measurement of multiple functional and phenotypic parameters including cytokine bead arrays, across complex cellular systems.

SPR — Surface Plasmon Resonance

Real-time kinetic understanding of target engagement — kon, koff, KD — in biochemical and cell-based formats.

Reporter Gene Assays

Fast, quantitative functional readout using stable reporter cell lines (e.g. NF-κB-Luc) for high-throughput pathway activation and inhibition screening.

Cell Binding Kinetics

Potency and target engagement characterisation in biologically relevant cellular systems.

Technologies

Flow Cytometry

Multiparametric, high-throughput analysis enabling simultaneous measurement of multiple functional and phenotypic parameters including cytokine bead arrays, across complex cellular systems.

SPR — Surface Plasmon Resonance

Real-time kinetic understanding of target engagement — kon, koff, KD — in biochemical and cell-based formats.

Reporter Gene Assays

Fast, quantitative functional readout using stable reporter cell lines (e.g. NF-κB-Luc) for high-throughput pathway activation and inhibition screening.

Cell Binding Kinetics

Potency and target engagement characterisation in biologically relevant cellular systems.

Custom and off-the-shelf  

Off-the-shelf validated assays are deployed where standard formats address the question. Where the target biology or mechanism requires a tailored approach, FairJourney Bio scientists design the assay from the ground up — acting as an extension of the partner team.


  • Off-the-shelf: validated, fast deployment for standard functional endpoints

  • Custom: tailored designs for unique mechanisms and novel antibody formats

  • Extensive cell line portfolio for engagement in biologically relevant systems

Custom and off-the-shelf  

Off-the-shelf validated assays are deployed where standard formats address the question. Where the target biology or mechanism requires a tailored approach, FairJourney Bio scientists design the assay from the ground up — acting as an extension of the partner team.


  • Off-the-shelf: validated, fast deployment for standard functional endpoints

  • Custom: tailored designs for unique mechanisms and novel antibody formats

  • Extensive cell line portfolio for engagement in biologically relevant systems

Custom and off-the-shelf  

Off-the-shelf validated assays are deployed where standard formats address the question. Where the target biology or mechanism requires a tailored approach, FairJourney Bio scientists design the assay from the ground up — acting as an extension of the partner team.


  • Off-the-shelf: validated, fast deployment for standard functional endpoints

  • Custom: tailored designs for unique mechanisms and novel antibody formats

  • Extensive cell line portfolio for engagement in biologically relevant systems

Scientist-Led. Decision-Focused.

Process & Deliverables   

Every FunctionInsight Assays engagement starts with the biological question. FairJourney Bio scientists act as an extension of the partner team — designing the assay strategy around what your molecule needs to demonstrate, then delivering results with the interpretation needed to make confident decisions.

Scientist-Led. Decision-Focused.

Process & Deliverables   

Every FunctionInsight Assays engagement starts with the biological question. FairJourney Bio scientists act as an extension of the partner team — designing the assay strategy around what your molecule needs to demonstrate, then delivering results with the interpretation needed to make confident decisions.

Scientist-Led. Decision-Focused.

Process & Deliverables   

Every FunctionInsight Assays engagement starts with the biological question. FairJourney Bio scientists act as an extension of the partner team — designing the assay strategy around what your molecule needs to demonstrate, then delivering results with the interpretation needed to make confident decisions.

Access is exclusive. No licensing. No competitor access. Lineage gatekeeping protects your programme's IP throughout.

Workflow

01

Understand what functional behaviour matters most

The engagement begins with a scientific discussion of the molecule's Target Product Profile, mechanism of action hypothesis, and the specific functional questions at this stage of development.

02

Design the optimal assay strategy

FairJourney Bio scientists select or develop the assay formats, cell systems, readouts, and controls that will generate the most informative data for the specific biological question.

03

Generate reliable, reproducible data

Assays are executed using proven technologies — flow cytometry, SPR, reporter gene assays, and cell binding kinetics — analysed by expert scientists.

04

Deliver results with scientific interpretation

Results are delivered as scientifically interpreted reports — with conclusions and clear guidance on which candidates to advance, which to deprioritise, and what the next question should be.

What You Receive

  • Functional assay data across all agreed readouts

  • Dose-response curves and potency values (IC50, EC50) where applicable

  • Candidate ranking and down-selection data

  • Scientific interpretation report: what the data means for your programme

  • Data in agreed formats for integration into partner systems

Why partners choose FunctionInsight Assays  

Assays

the widest panel, from target binding to Fab/VHH/scFv- and Fc-mediated functions

Customisation

off-the-shelf to fully tailored designs for unique mechanisms

Speed

automated, high-throughput systems enabling 280+ antibody screening campaigns

Diversity

extensive cell line portfolio for biologically relevant engagement

Expertise

scientists acting as an extension of your team across integrated programmes

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