>50
overexpressing cell lines generated
>40
reporter and knockout cell lines
>30
cell lines available in-house for immediate access
Cells transferred to partner
CellCraft Solutions provides the cellular infrastructure that powers functional screening across the entire discovery workflow — from target validation and hit screening through to lead selection and functional characterisation.
Target Validation — design of the right cell line for the right purpose
Hit Discovery — reliable, high-quality cell line target expression for efficient screening
Lead Selection / Development — cell model generation for functional characterisation
Overexpression
Hyperactive Transposase
Stable, high-level target expression by integration into highly active genomic loci — enabling robust, reproducible functional screening across large antibody panels.
High stable expression by transposon integration into highly active loci
High success rate with challenging targets: ion channels and GPCRs
Expression level validated by flow cytometry and functional assay confirmation
Licensed technology validated in the literature in >100 scientific publications
Best for: Cell-based immunizations, antibody library selections, primary screening against cell surface-expressed targets; functional assay development where consistent, high-level target expression is required.
Reporter
Hyperactive Transposase
Regulatory elements associated to Luciferase, GFP, or other reporter genes, enabling quantitative, high-throughput readout of pathway activation or inhibition in cell-based functional assays.
Transcriptional regulatory elements to exploit different signalling pathways such as NF-κB, NFAT, AP-1, STAT1/3/5 family, SMADs, CREB, among others
Single-cell clone generation with high signal sensitivity, reproducibility and stability
Designed for screening and characterization of pathway activation, blocking, or inhibition and FACS-based single B-cell discovery
Best for: antagonist and agonist screening; high-throughput functional characterisation of large antibody panels within regular discovery platform or potency assessment with dose-response characterization for IND support
Knockout
High-precision Nuclease Technology
High-efficiency gene knockout with virtually no possibility of having off-targets – generating clean, well-validated negative controls and target-null backgrounds for mechanistic and selectivity studies.
Well-validated licensed technology
Large deletions achieved with high knockout percentage
High precision with limited-to-no off-target events
Best for: Generating isogenic knockout cells for antibody library selections using endogenously expressing cell lines; validating target-specific effects in functional assays; Other studies requiring target-null cellular background.
Workflow
01
Stable pool generation
Synthetic genes or sgRNAs are designed, ordered and cloned in appropriate plasmids from an in-house library of over 50 vector backbones.
Transfection and initial selection take place to generate the stable pools Expression or functionality is tested both 24h after transfection and at stable pool stage.
Pools can be used directly for antibody library selections and early-stage screening where single-cell clone homogeneity is not yet required.
02
Single-cell clone selection
Single-cell FACSorting to obtain desired expression level (e.g. low and high-expressing clones).
Up to 30 clones (overexpression) or 60 clones (reporter/knockout) are screened by expression levels or functional performance.
03
Single-cell clone final characterization
Freeze-thaw viability tested. Target overexpression levels confirmed by flow cytometry.
Reporter cell lines validated for signal induction and fold-change upon stimulation and EC50/IC50 calculated for reference antibodies.
Both are tested for stability expression and performance over 2-weeks. Knockout cell lines confirmed by sequencing and target loss-of-expression.
What You Receive
5 vials per pool or clone selection cell bank
Datasheet with QC data and cell culture and developed assay conditions
Overview report (PPT format) containing raw and processed characterisation data
Expression validation data: flow cytometry, Western blot, or functional assay as appropriate
Transfer of cells to partner for research use — no associated fees
Cold storage and BioLogistics: cells can be shipped to the partner or stored at −196°C (liquid nitrogen) at FairJourney Bio
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