Single B-Cell Screening

Single B-Cell screening reads the antibody response the way the immune system built it: as natively paired heavy and light chains, recovered directly from the cells that produced them. FairJourney Bio screens the full immune repertoire, memory B-Cells and plasma cells together, to recover diverse, functional antibodies that single-population methods leave behind.

Discuss a single B-Cell campaign

Single B-Cell screening reads the antibody response the way the immune system built it: as natively paired heavy and light chains, recovered directly from the cells that produced them. FairJourney Bio screens the full immune repertoire, memory B-Cells and plasma cells together, to recover diverse, functional antibodies that single-population methods leave behind.

Discuss a single B-Cell campaign

Single B-Cell screening reads the antibody response the way the immune system built it: as natively paired heavy and light chains, recovered directly from the cells that produced them. FairJourney Bio screens the full immune repertoire, memory B-Cells and plasma cells together, to recover diverse, functional antibodies that single-population methods leave behind.

Discuss a single B-Cell campaign

A dual-engine approach

FairJourney Bio runs two screening engines in parallel, each matched to a different B-Cell population, so a single immunisation is read in full, and in depth.

Discuss your storage and logistics requirements with our team

A dual-engine approach

FairJourney Bio runs two screening engines in parallel, each matched to a different B-Cell population, so a single immunisation is read in full, and in depth.

Discuss your storage and logistics requirements with our team

A dual-engine approach

FairJourney Bio runs two screening engines in parallel, each matched to a different B-Cell population, so a single immunisation is read in full, and in depth.

Discuss your storage and logistics requirements with our team

Engine 1

Imaging spectral sorting (memory B-Cells)

Memory B-Cells are sorted on direct visual evidence of antigen binding. Spectral unmixing removes cellular autofluorescence, so true binders are separated from background rather than buried in it, and real-time imaging confirms where binding occurs and links each sorted cell to its phenotypic data. The outcome is high confidence in monoclonality before a single sequence is recovered.

Engine 1

Imaging spectral sorting (memory B-Cells)

Memory B-Cells are sorted on direct visual evidence of antigen binding. Spectral unmixing removes cellular autofluorescence, so true binders are separated from background rather than buried in it, and real-time imaging confirms where binding occurs and links each sorted cell to its phenotypic data. The outcome is high confidence in monoclonality before a single sequence is recovered.

Engine 1

Imaging spectral sorting (memory B-Cells)

Memory B-Cells are sorted on direct visual evidence of antigen binding. Spectral unmixing removes cellular autofluorescence, so true binders are separated from background rather than buried in it, and real-time imaging confirms where binding occurs and links each sorted cell to its phenotypic data. The outcome is high confidence in monoclonality before a single sequence is recovered.

Engine 2

Microfluidic droplet sorting (plasma cells)

Plasma cells secrete antibody but do not display it, so they are screened in single-cell microreactors that capture each cell together with its secreted IgG. More than one million plasma cells can be screened in a single run, and the assay is multiplexed: IgG secretion, target specificity, and function (blocking, agonism, antagonism, internalisation) are read at the same time. Functional candidates are selected directly, not inferred after the fact.

Both engines recover natively paired VH–VL sequences, across multiple species including human donors and transgenic mice, at 106–107 B-Cells per run.

Engine 2

Microfluidic droplet sorting (plasma cells)

Plasma cells secrete antibody but do not display it, so they are screened in single-cell microreactors that capture each cell together with its secreted IgG. More than one million plasma cells can be screened in a single run, and the assay is multiplexed: IgG secretion, target specificity, and function (blocking, agonism, antagonism, internalisation) are read at the same time. Functional candidates are selected directly, not inferred after the fact.

Both engines recover natively paired VH–VL sequences, across multiple species including human donors and transgenic mice, at 106–107 B-Cells per run.

Engine 2

Microfluidic droplet sorting (plasma cells)

Plasma cells secrete antibody but do not display it, so they are screened in single-cell microreactors that capture each cell together with its secreted IgG. More than one million plasma cells can be screened in a single run, and the assay is multiplexed: IgG secretion, target specificity, and function (blocking, agonism, antagonism, internalisation) are read at the same time. Functional candidates are selected directly, not inferred after the fact.

Both engines recover natively paired VH–VL sequences, across multiple species including human donors and transgenic mice, at 106–107 B-Cells per run.

Where single B-cell screening sits in discovery

Single B-cell screening is one of several sources of diversity FairJourney Bio brings to hit discovery, alongside phage, mammalian, and yeast display within MultiPath Discovery. Hits move straight into functional work through FunctionInsight Assays and FullSpectrum Characterization, and into optimisation through DeepDesign Engineering, inside one integrated workflow that runs from target validation to IND. Nothing is handed to a third party, and no diversity is lost at the seams.

Where single B-cell screening sits in discovery

Single B-cell screening is one of several sources of diversity FairJourney Bio brings to hit discovery, alongside phage, mammalian, and yeast display within MultiPath Discovery. Hits move straight into functional work through FunctionInsight Assays and FullSpectrum Characterization, and into optimisation through DeepDesign Engineering, inside one integrated workflow that runs from target validation to IND. Nothing is handed to a third party, and no diversity is lost at the seams.

Where single B-cell screening sits in discovery

Single B-cell screening is one of several sources of diversity FairJourney Bio brings to hit discovery, alongside phage, mammalian, and yeast display within MultiPath Discovery. Hits move straight into functional work through FunctionInsight Assays and FullSpectrum Characterization, and into optimisation through DeepDesign Engineering, inside one integrated workflow that runs from target validation to IND. Nothing is handed to a third party, and no diversity is lost at the seams.

From repertoire to qualified binders

In a recent anti-OX40 campaign, the imaging spectral engine screened 6.3 million B-Cells in a single run and returned 17 unique, strong binders from three plates. That is the memory arm on its own. Run in parallel with the plasma-cell engine, the same immunisation is mined for the full response.

The complete dual-engine case study, timelines, and how a campaign is run live on the B-Cell Navigator platform.

See the B-Cell Navigator platform

From repertoire to qualified binders

In a recent anti-OX40 campaign, the imaging spectral engine screened 6.3 million B-Cells in a single run and returned 17 unique, strong binders from three plates. That is the memory arm on its own. Run in parallel with the plasma-cell engine, the same immunisation is mined for the full response.

The complete dual-engine case study, timelines, and how a campaign is run live on the B-Cell Navigator platform.

See the B-Cell Navigator platform

From repertoire to qualified binders

In a recent anti-OX40 campaign, the imaging spectral engine screened 6.3 million B-Cells in a single run and returned 17 unique, strong binders from three plates. That is the memory arm on its own. Run in parallel with the plasma-cell engine, the same immunisation is mined for the full response.

The complete dual-engine case study, timelines, and how a campaign is run live on the B-Cell Navigator platform.

See the B-Cell Navigator platform

Start a single B-cell campaign

Planning discovery that needs the full immune repertoire, not a slice of it? Tell us the target and we will scope the right approach. FJB now also offers this service in combination with AbTherx’s Atlas™ Full human antibody transgenic mice – for fully human antibody discovery end to end.

Talk to our team

Explore B-Cell Navigator

Start a single B-cell campaign

Planning discovery that needs the full immune repertoire, not a slice of it? Tell us the target and we will scope the right approach. FJB now also offers this service in combination with AbTherx’s Atlas™ Full human antibody transgenic mice – for fully human antibody discovery end to end.

Talk to our team

Explore B-Cell Navigator

Start a single B-cell campaign

Planning discovery that needs the full immune repertoire, not a slice of it? Tell us the target and we will scope the right approach. FJB now also offers this service in combination with AbTherx’s Atlas™ Full human antibody transgenic mice – for fully human antibody discovery end to end.

Talk to our team

Explore B-Cell Navigator

Single B-Cell Screening

Why screen the whole repertoire

Lost diversity

Missed rare clones

Binders that aren’t leads

A dual-engine approach

A dual-engine approach

A dual-engine approach

Engine 1

Imaging spectral sorting (memory B-Cells)

Engine 1

Imaging spectral sorting (memory B-Cells)

Engine 1

Imaging spectral sorting (memory B-Cells)

Engine 2

Microfluidic droplet sorting (plasma cells)

Engine 2

Microfluidic droplet sorting (plasma cells)

Engine 2

Microfluidic droplet sorting (plasma cells)

Where single B-cell screening sits in discovery

Where single B-cell screening sits in discovery

Where single B-cell screening sits in discovery

From repertoire to qualified binders

From repertoire to qualified binders

From repertoire to qualified binders

Start a single B-cell campaign

Start a single B-cell campaign

Start a single B-cell campaign